Please use this identifier to cite or link to this item:
Title: Sleep deprivation inhibits expression of NADPH-d and NOS while activating microglia and astroglia in the rat hippocampus
Authors: Hsu, J.-C.
Lee, Y.-S.
Chang, C.-N.
Chuang, H.-L.
Ling, E.-A. 
Lan, C.-T.
Keywords: CNS
Nitric oxide
Western blot
Issue Date: 2003
Citation: Hsu, J.-C., Lee, Y.-S., Chang, C.-N., Chuang, H.-L., Ling, E.-A., Lan, C.-T. (2003). Sleep deprivation inhibits expression of NADPH-d and NOS while activating microglia and astroglia in the rat hippocampus. Cells Tissues Organs 173 (4) : 242-254. ScholarBank@NUS Repository.
Abstract: This study investigated the expression of nitric oxide (NO)-synthesizing enzymes and the glial reaction in the rat hippocampal formation following sleep deprivation for 5 days. Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) reactivity was markedly reduced in the hippocampal CA1, CA2 and CA3 sectors as well as in the dentate gyrus, suggesting a suppression of NO production in these areas. Microglial cells were hypertrophic and showed an up-regulation of complement type 3 receptors as determined by antibody OX-42. However, expression of major histocompatibility complex class I and II antigens, and antigen of monocyte/macrophage lineage marked by OX-18, OX-6 and ED1, respectively, was undetected. Astrocytes also displayed hypertrophied processes with enhanced glial fibrillary acidic protein (GFAP) immunoreactivity. Western blots of hippocampal tissues corroborated the above-mentioned morphological findings in that expression of NO-synthase (NOS) was decreased while that of OX-42 and GFAP was increased in the sleep-deprived rats. Since NO is thought to be involved in memory consolidation processes in the hippocampus during sleep, the inhibition of NADPH-d and NOS reactivities may account for the memory decline after long-term sleep deprivation. The concomitant reactions in microglia and astrocytes suggest the involvement of these cells in the deleterious effect of prolonged sleep deprivation. Copyright © 2003 S. Karger AG, Basel.
Source Title: Cells Tissues Organs
ISSN: 14226405
DOI: 10.1159/000070380
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Apr 24, 2019


checked on Apr 16, 2019

Page view(s)

checked on Apr 22, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.