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Title: Injectable mesenchymal stem cell therapy for large cartilage defects - A porcine model
Authors: Lee, K.B.L.
Hui, J.H.P. 
Im, C.S.
Ardany, L.
Eng, H.L.
Keywords: Cartilage defects
Hyaluronic acid
Mesenchymal stem cells
Minimally invasive
Issue Date: Nov-2007
Citation: Lee, K.B.L., Hui, J.H.P., Im, C.S., Ardany, L., Eng, H.L. (2007-11). Injectable mesenchymal stem cell therapy for large cartilage defects - A porcine model. Stem Cells 25 (11) : 2964-2971. ScholarBank@NUS Repository.
Abstract: Current techniques in biological resurfacing of cartilage defects require an open arthrotomy or arthroscopy and involve the direct transplantation of isolated cells and/or scaffolds or whole tissue grafts with chondrogenic potential onto the cartilage defect. Our study investigates the possibility of direct intra-articular injection of mesenchymal stem cells suspended in hyaluronic acid (HA) as an alternative to the much more invasive methods currently available. A partial-thickness (without penetration of the subchondral bone) cartilage defect was created in the medial femoral condyle of an adult minipig. Mesenchymal stem cells from the iliac crest marrow of the same pig harvested in a separate procedure and suspended in 2 milliliters of hylan G-F 20 (Synvisc) were injected intra-articularly after the creation of the defect. This was followed by two more injections of hylan G-F 20 (HA) at weekly intervals. Either saline or HA was injected into the knees of the controls. The pigs were sacrificed at 6 and 12 weeks for morphological and histological analysis. The cell-treated groups showed improved cartilage healing both histologically and morphologically at 6 and 12 weeks compared with both controls. The use of intra-articular injections of mesenchymal stem cells suspended in HA is a viable option for treating large cartilage defects. This would be further explored in clinical trials. ©AlphaMed Press.
Source Title: Stem Cells
ISSN: 10665099
DOI: 10.1634/stemcells.2006-0311
Appears in Collections:Staff Publications

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