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Title: Profiling of hepatocellular proteins by 1D PAGE-MALDI/MS/MS in a rat heat stress model
Authors: Rajaseger, G.
Lim, C.L.
Lee, K.W.
Arjunan, P. 
Jia, L.
Moochhala, S.
Keywords: 1D PAGE
Heat stress
Hepatocellular proteins
Polyacrylamide gel electrophoresis
Rat model
Issue Date: 2006
Citation: Rajaseger, G., Lim, C.L., Lee, K.W., Arjunan, P., Jia, L., Moochhala, S. (2006). Profiling of hepatocellular proteins by 1D PAGE-MALDI/MS/MS in a rat heat stress model. Frontiers in Bioscience 11 (SUPPL. 3) : 2924-2928. ScholarBank@NUS Repository.
Abstract: Heat induced complications cause an increase in a large number of proteins which play a role in diverse pathways during heat shock. A detailed characterization of these proteins is essential for understanding the molecular mechanisms involved in heat stroke. In this report, the proteins present in rat liver were compared at 37 °C (control) and at core temperature (Tc) 42 °C (heat stress) by 1D PAGE and MALDI/MS/MS. Among proteins identified in the sample after heat stress are dimethyglycine dehydrogenase, transketolase, carboxylic ester hydrolase, pyruvate kinase, L-type pyruvate kinase, arginosuccinate synthetase; fumarylacetoacetate hydrolase and peptidylpropyl isomerase A. These findings show that analysis of large scale proteins by MALDI/MS/MS provides a better understanding of the molecular mechanisms associated with heat shock. The resolution of proteins examined by 1D-PAGE was less than that obtained with 2D-PAGE. More specifically, 2D-PAGE allows better identification of low molecular weight proteins that can not be resolved by 1D-PAGE.
Source Title: Frontiers in Bioscience
ISSN: 10939946
Appears in Collections:Staff Publications

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