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|Title:||Inhibition of various isoforms of rat liver glutathione S-transferases by tannic acid and butein||Authors:||Zhang, K.
|Issue Date:||Jul-1997||Citation:||Zhang, K.,Mack, P.,Wong, K.P. (1997-07). Inhibition of various isoforms of rat liver glutathione S-transferases by tannic acid and butein. Biochemistry and Molecular Biology International 42 (4) : 701-710. ScholarBank@NUS Repository.||Abstract:||Glutathione S-transferases (EC.188.8.131.52, GSTs) were purified from rat liver by S-hexylglutathione affinity chromatography and six isoforms, namely C-1, C-2, C-3, C-4, A-2 and A-1, were isolated by CM-cellulose and DEAF-cellulose ion-exchange columns. Tannic acid and butein showed varying degrees of inhibition on the six individual GST isoforms. When 1-chloro-2,4-dinitrobenzene (CDNB) was used as a substrate, butein exerted significantly more potent inhibition on the cationic isoforms C-2, C-3 and C-4 with IC 50 values of 6.8, 8.5 and 8.0 μM respectively. All the isoforms showed lower activity towards p-nitrobenzyl chloride when compared to CDNB and inhibition of the p-nitrobenzyl chloride-activity by tannic acid and butein was also weaker. The inhibitory effects of tannic acid and butein on each isoform decreased generally with increasing pH in the range of 6.0 to 8.0. The optimum pHs for inhibitions by tannic acid and butein on the six individual isoforms lie in the pH range of 6.0 to 6.5.||Source Title:||Biochemistry and Molecular Biology International||URI:||http://scholarbank.nus.edu.sg/handle/10635/107769||ISSN:||10399712|
|Appears in Collections:||Staff Publications|
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