Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/107594
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dc.titleIntracytoplasmic injection of human sperm into frozen-thawed hamster oocytes
dc.contributor.authorAhmadi, A.
dc.contributor.authorBongso, A.
dc.contributor.authorNg, S.C.
dc.date.accessioned2014-11-10T08:03:54Z
dc.date.available2014-11-10T08:03:54Z
dc.date.issued1996
dc.identifier.citationAhmadi, A.,Bongso, A.,Ng, S.C. (1996). Intracytoplasmic injection of human sperm into frozen-thawed hamster oocytes. Medical Science Research 24 (10) : 693-694. ScholarBank@NUS Repository.
dc.identifier.issn02698951
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/107594
dc.description.abstractSuperovulated hamster oocytes were frozen by 2 M DMSO. 89% (499/561) of the oocytes survived freezing and thawing. A hamster-ICSI assay was performed to evaluate the ability of frozen-thawed oocytes to develop male pronuclei. 46% (58/127) of the oocytes had male pronuclear formation following injection of single human sperm into the cytoplasm of frozen-thawed oocytes. There was no significant difference in male pronuclear formation as compared with fresh oocytes. This study shows that hamster oocytes conserved their ability to support human sperm head decondensation and male pronuclear formation following freezing-thawing.
dc.sourceScopus
dc.subjectCryopreservation
dc.subjectHamster-ICSI assay
dc.subjectIntracytoplasmic sperm injection (ICSI)
dc.subjectOocyte
dc.subjectSperm
dc.typeArticle
dc.contributor.departmentOBSTETRICS & GYNAECOLOGY
dc.description.sourcetitleMedical Science Research
dc.description.volume24
dc.description.issue10
dc.description.page693-694
dc.description.codenMSCRE
dc.identifier.isiutNOT_IN_WOS
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