Please use this identifier to cite or link to this item:
Title: Intracytoplasmic injection of human sperm into frozen-thawed hamster oocytes
Authors: Ahmadi, A.
Bongso, A. 
Ng, S.C. 
Keywords: Cryopreservation
Hamster-ICSI assay
Intracytoplasmic sperm injection (ICSI)
Issue Date: 1996
Citation: Ahmadi, A.,Bongso, A.,Ng, S.C. (1996). Intracytoplasmic injection of human sperm into frozen-thawed hamster oocytes. Medical Science Research 24 (10) : 693-694. ScholarBank@NUS Repository.
Abstract: Superovulated hamster oocytes were frozen by 2 M DMSO. 89% (499/561) of the oocytes survived freezing and thawing. A hamster-ICSI assay was performed to evaluate the ability of frozen-thawed oocytes to develop male pronuclei. 46% (58/127) of the oocytes had male pronuclear formation following injection of single human sperm into the cytoplasm of frozen-thawed oocytes. There was no significant difference in male pronuclear formation as compared with fresh oocytes. This study shows that hamster oocytes conserved their ability to support human sperm head decondensation and male pronuclear formation following freezing-thawing.
Source Title: Medical Science Research
ISSN: 02698951
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on May 18, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.