Please use this identifier to cite or link to this item: https://doi.org/10.1017/S0967199406003947
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dc.titleSomatic cell nuclear transfer using transported in vitro-matured oocytes in cynomolgus monkey
dc.contributor.authorChen, N.
dc.contributor.authorLiow, S.-L.
dc.contributor.authorBin Abdullah, R.
dc.contributor.authorKhadijah Wan Embong, W.
dc.contributor.authorYip, W.-Y.
dc.contributor.authorTan, L.-G.
dc.contributor.authorTong, G.-Q.
dc.contributor.authorNg, S.-C.
dc.date.accessioned2014-11-06T08:59:41Z
dc.date.available2014-11-06T08:59:41Z
dc.date.issued2007-02
dc.identifier.citationChen, N., Liow, S.-L., Bin Abdullah, R., Khadijah Wan Embong, W., Yip, W.-Y., Tan, L.-G., Tong, G.-Q., Ng, S.-C. (2007-02). Somatic cell nuclear transfer using transported in vitro-matured oocytes in cynomolgus monkey. Zygote 15 (1) : 25-33. ScholarBank@NUS Repository. https://doi.org/10.1017/S0967199406003947
dc.identifier.issn09671994
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/107570
dc.description.abstractSomatic cell nuclear transfer (SCNT) is not successful so far in non-human primates. The objective of this study was to investigate the effects of stimulation cycles (first and repeat) on oocyte retrieval and in vitro maturation (IVM) and to evaluate the effects of stimulation cycles and donor cell type (cumulus and fetal skin fibroblasts) on efficiency of SCNT with transported IVM oocytes. In this study, 369 immature oocytes were collected laparoscopically at 24 h following human chorionic gonadotrophin (hCG) treatment from 12 cynomolgus macaque (Macaca fascicularis) in 24 stimulation cycles, and shipped in pre-equilibrated IVM medium for a 5 h journey, placed in a dry portable incubator (37°C) without CO 2 supplement. A total of 70.6% (247/350) of immature oocytes reached metaphase II (MII) stage at 36 h after hCG administration, MII spindle could be seen clearly in 80.6% (104/129) of matured IVM oocytes under polarized microscopy. A total of 50.0% (37/74) of reconstructive SCNT embryos cleaved after activation; after cleavage, 37.8% (14/37) developed to the 8-cell stage and 8.1% (3/37) developed to morula, but unfortunately none developed to the blastocyst stage. Many more oocytes could be retrieved per cycle from monkeys in the first cycle than in repeated cycles (19.1 vs. 11.7, p<0.05). There were no significant differences in the maturation rate (70.0 vs. 71.4%, p>0.05) and MII spindle rate under polarized microscopy (76.4 vs. 86.0%, p>0.05) between the first and repeat cycles. There were also no significant differences in the cleavage rate, and the 4-cell, 8-cell and morula development rate of SCNT embryos between the first and repeat cycles. When fibroblast cells and cumulus cells were used as the donor cells for SCNT, first cleavage rate was not significantly different, but 4-cell (50.0 vs. 88.9%, p<0.05) and 8-cell (0 vs. 51.9%, p<0.01) development rate were significantly lower for the former. In conclusion, the number of stimulation cycles has a significant effect on oocyte retrieval, but has no effect on maturation and SCNT embryo development; however, different donor cell types (cumulus and fibroblast) resulted in different developmental potentials of SCNT embryos. © 2007 Cambridge University Press.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1017/S0967199406003947
dc.sourceScopus
dc.subjectCloning
dc.subjectIn vitro maturation
dc.subjectMonkey
dc.subjectNuclear transfer
dc.subjectOocytes
dc.subjectTransport
dc.typeArticle
dc.contributor.departmentOBSTETRICS & GYNAECOLOGY
dc.description.doi10.1017/S0967199406003947
dc.description.sourcetitleZygote
dc.description.volume15
dc.description.issue1
dc.description.page25-33
dc.description.codenZYGOE
dc.identifier.isiut000244258600004
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