Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/107491
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dc.titleCalcitonin gene-related peptide protects cultured rat gastric mucosal cells
dc.contributor.authorTu, Y.
dc.contributor.authorKang, J.-Y.
dc.date.accessioned2014-11-06T08:25:05Z
dc.date.available2014-11-06T08:25:05Z
dc.date.issued1998
dc.identifier.citationTu, Y.,Kang, J.-Y. (1998). Calcitonin gene-related peptide protects cultured rat gastric mucosal cells. European Journal of Gastroenterology and Hepatology 10 (4) : 317-324. ScholarBank@NUS Repository.
dc.identifier.issn0954691X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/107491
dc.description.abstractCapsaicin exerts its gastroprotective effect by stimulating primary afferent neurons, releasing calcitonin gene-related peptide (CGRP), which in turn increases gastric blood flow. In this work, the effects of capsaicin, rat α-CGRP, and relative peptides hCGRP8-37 and β-hCGRP, and substance P on cultured gastric mucosal cells independent of neural and vascular mechanisms were studied. Damage was produced by indomethacin, ethanol or taurocholate 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenybromide and trypan blue exclusion tests were used to assess viability of the cultured cells. Capsaicin administration alone did not injure gastric cells. However, capsaicin pretreatment potentiated the damaging effect of indomethacin and ethanol. In the sodium taurocholate model, capsaicin slightly protected the cells against injury, α-rCGRP was protective against indomethacin, ethanol and taurocholate in a dose-dependent manner, hCGRP8-37 and β-hCGRP both dose-dependently prevented injury caused by indomethacin at concentrations about eight times higher than that of α-rCGRP, but substance P was ineffective in the three different damage models. A combination of α-CGRP and hCGRP8-37 was also protective against indomethacin damage to a similar extent as use of either agent alone. The defence mechanism of capsaicin against gastric cell injury may in part be mediated by a direct effect of CGRP on gastric mucosal cells, in addition to effects dependent on neural and vascular mechanisms, hCGRP8-37 has no antagonist effect against CGRP in this model, suggesting that CGRP receptors in this model may be different from those in other tissues.
dc.sourceScopus
dc.subjectCalcitonin gene-related peptide
dc.subjectCapsaicin
dc.subjectCultured gastric mucosal cells
dc.subjectSubstance P
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.contributor.departmentMEDICINE
dc.description.sourcetitleEuropean Journal of Gastroenterology and Hepatology
dc.description.volume10
dc.description.issue4
dc.description.page317-324
dc.description.codenEJGHE
dc.identifier.isiutNOT_IN_WOS
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