Please use this identifier to cite or link to this item:
Title: Preparation of self-assembled monolayers containing anthryl groups toward hybridization of nucleotides
Authors: Nakamura, F.
Mitsui, K.
Hara, M.
Kraemer, S.
Mittler, S.
Knoll, W. 
Issue Date: 8-Jul-2003
Citation: Nakamura, F., Mitsui, K., Hara, M., Kraemer, S., Mittler, S., Knoll, W. (2003-07-08). Preparation of self-assembled monolayers containing anthryl groups toward hybridization of nucleotides. Langmuir 19 (14) : 5823-5829. ScholarBank@NUS Repository.
Abstract: To realize immobilization and hybridization of nucleotides, self-assembled monolayers (SAMs) containing anthryl groups which interact with nucleotides were prepared on a gold substrate. We used an unsymmetric disulfide containing an anthryl group (Anth-s-s-C8), which can form a SAM on a gold substrate, to immobilize nucleotides on the gold surface via the intercalation between DNA and anthryl groups. The SAM was characterized by time-of-flight mass spectroscopy. The interaction between polynucleotides and the SAM and hybridization were investigated by surface plasmon resonance. The surface coverage of anthryl groups on the SAM could be controlled by mixing Anth-s-s-C8 and 11-mercapto-1-undecanol in solution at various ratios. The efficient hybridization of polynucleotides could be realized by controlling 30% of Anth-s-s-C8 on the surface. This method can be applied to oligonucleotides, and the efficiency of hybridization is enhanced by introducing the double-stranded portion in the middle.
Source Title: Langmuir
ISSN: 07437463
DOI: 10.1021/la020952d
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jun 16, 2021


checked on Jun 8, 2021

Page view(s)

checked on Jun 7, 2021

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.