Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/106928
Title: Two major groups of vitellogenin cDNA clones from Oreochromis aureus (steindachner)
Authors: Lee, B.H.
Lim, E.H. 
Lam, T.J. 
Ding, J.L. 
Issue Date: 1994
Citation: Lee, B.H.,Lim, E.H.,Lam, T.J.,Ding, J.L. (1994). Two major groups of vitellogenin cDNA clones from Oreochromis aureus (steindachner). Biochemistry and Molecular Biology International 34 (1) : 75-83. ScholarBank@NUS Repository.
Abstract: cDNA libraries were constructed using livers of male and female Oreochromis aureus that were stimulated with estradiol. Restriction map analyses of vitellogenin (Vg) cDNA clones indicated 4 subgroups with related restriction patterns, represented by pOAVg2 (from female) and pOAVg50, 71 and 87 (from male) fish. With the exception of pOAVg2, all other clones obtained from female fish resembled pOAVg50. There are no internal repeats within these cDNA clones. Southern blot cross-hybridisations distinguished pOAVg87 (group A) from pOAVg2, 50 and 71 (group B). The existence of these 2 major groups of Vg cDNAs was further confirmed by dot blot hybridisations and hybrid melting assays under varying stringencies. The difference of melting temperature, T(m) between the 2 groups suggests a 7% intraspecific divergence in the Vg gene family.
Source Title: Biochemistry and Molecular Biology International
URI: http://scholarbank.nus.edu.sg/handle/10635/106928
ISSN: 10399712
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

42
checked on Sep 21, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.