Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF00004312
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dc.titleSome properties of the intestinal proteases of the rabbitfish, Siganus canaliculatus (Park)
dc.contributor.authorSabapathy, U.
dc.contributor.authorTeo, L.-H.
dc.date.accessioned2014-10-29T07:55:33Z
dc.date.available2014-10-29T07:55:33Z
dc.date.issued1995-06
dc.identifier.citationSabapathy, U., Teo, L.-H. (1995-06). Some properties of the intestinal proteases of the rabbitfish, Siganus canaliculatus (Park). Fish Physiology and Biochemistry 14 (3) : 215-221. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00004312
dc.identifier.issn09201742
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/106894
dc.description.abstractSome properties of the intestinal proteases of the rabbitfish were examined. At 25°C, both trypsin and chymotrypsin showed pH optima of 8.0. Leucine aminopeptidase, however, displayed maximum activity in the pH range, 7.0-9.0. Leucine aminopeptidase had the highest optimum temperature (60°C), and chymotrypsin, the lowest (30°C). The optimum temperature of trypsin was 55°C. The activation energy, Ea, was found to be 8.24 for trypsin and 8.50 kcal mol-1 for chymotrypsin. The Ea for leucine aminopeptidase was 6.29 kcal mol-1 above 40°C and 1.73 kcal mol-1 below 40°C. Substrate concentration-velocity plots showed that all three enzymes followed Michaelis-Menten kinetics; the Km and Vmax were estimated for the three enzymes. The effects of various protease inhibitors on enzyme activity were also examined and confirmed the protease classes to which each enzyme belonged. The three proteases examined have similar properties to proteases in other fishes. © 1995 Kugler Publications.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/BF00004312
dc.sourceScopus
dc.subjectchymotrypsin
dc.subjectdigestion
dc.subjectfish
dc.subjectleucine aminopeptidase
dc.subjectproteases
dc.subjecttrypsin
dc.typeArticle
dc.contributor.departmentZOOLOGY
dc.description.doi10.1007/BF00004312
dc.description.sourcetitleFish Physiology and Biochemistry
dc.description.volume14
dc.description.issue3
dc.description.page215-221
dc.description.codenFPBIE
dc.identifier.isiutA1995RE15700004
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