Please use this identifier to cite or link to this item: https://doi.org/10.1074/jbc.271.8.4106
Title: Skin antifreeze protein genes of the winter flounder, Pleuronectes americanus, encode distinct and active polypeptides without the secretory signal and prosequences
Authors: Gong, Z. 
Ewart, K.V.
Hu, Z.
Fletcher, G.L.
Hew, C.L.
Issue Date: 23-Feb-1996
Citation: Gong, Z.,Ewart, K.V.,Hu, Z.,Fletcher, G.L.,Hew, C.L. (1996-02-23). Skin antifreeze protein genes of the winter flounder, Pleuronectes americanus, encode distinct and active polypeptides without the secretory signal and prosequences. Journal of Biological Chemistry 271 (8) : 4106-4112. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.271.8.4106
Abstract: Distinct antifreeze polypeptides (AFP) were isolated from the skin of the winter flounder, Pleuronectes americanus, by gel filtration and reverse phase high performance liquid chromatography. In parallel, several cDNA clones were isolated from a skin cDNA library using a liver AFP cDNA probe. Both protein and DNA sequence analyses indicate that flounder skin contains several distinct but homologous alanine-rich AFPs. Although the skin type AFPs contain 11 similar amino acid repeats found in the secretory liver type AFPs, the skin type AFPs are mature polypeptides lacking both the signal and prosequences, indicating that they may function intracellularly. The skin type AFP is significantly less active in thermal hysteretic activity than the liver type AFP. Genomic Southern analysis indicates that like the liver type AFP genes, there are multiple copies (30-40 copies) of skin type AFP. Although the liver type AFP genes are specifically expressed in the liver and to a lesser extent in intestine, the skin type AFP genes are expressed in all tissues examined including the liver and abundantly in exterior tissues, i.e. skin, scales, fin, and gills, suggesting an important protecting role in these exterior tissues.
Source Title: Journal of Biological Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/106891
ISSN: 00219258
DOI: 10.1074/jbc.271.8.4106
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

75
checked on Sep 20, 2020

Page view(s)

87
checked on Sep 26, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.