Development of the dorsal root ganglion in a teleost, Oreochromis mossambicus (Peters)

Abstract

The precursor crest cells of the spinal dorsal root ganglia (DRG) in the tilapia, Oreochromis mossambicus, were analysed by HNK-1 antibody staining, scanning electron microscopy, and DiI labeling techniques. The ontogeny of the DRG was followed in the embryos and young fry of the fish. Neural crest cells which contribute to the formation of the DRG were observed to commence their migration in the trunk region after 40 hours postfertilization. They do not penetrate the somites but travel through the space between the neural tube and the somite. Crest cells destined to become the DRG accumulate at the midsomitic region where the ventral root exits. At 50 to 80 hours postfertilization, they differentiate and become bipolar sensory cells. The DRG continues to grow and develop right through hatching at 115 hours. During the early larval stages, crest cells accumulate around the ventral root and the DRG eventually fuses with the motor root, giving rise to a situation in which the DRG contains not only the sensory cells but also motor fibres. The mixed nature of the DRG was confirmed by HRP retrograde labeling. We believe that this is the first report in describing the formation of the DRG in a teleost.