Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/106769
Title: Cloning, characterisation and expression of vitellogenin gene of Oreochromis aureus (Teleostei, Cichlidae)
Authors: Ding, J.L. 
Ho, B.
Valotaire, Y.
LeGuellec, K.
Lim, E.H. 
Tay, S.P.
Lam, T.J. 
Issue Date: 1990
Citation: Ding, J.L.,Ho, B.,Valotaire, Y.,LeGuellec, K.,Lim, E.H.,Tay, S.P.,Lam, T.J. (1990). Cloning, characterisation and expression of vitellogenin gene of Oreochromis aureus (Teleostei, Cichlidae). Biochemistry International 20 (4) : 843-852. ScholarBank@NUS Repository.
Abstract: cDNA synthesized from liver Poly A+ RNA of estradiol-stimulated male Oreochromis areus was ligated to PTZ-19R and transformed into E. coli. TG-1 to yield 6 x 104 clones/ug DNA, of which 6% was vitellogenin positive. Restriction maps indicate 4 possible subgroups of vitellogenin cDNA, with homology, observed by Southern hybridisation, at a fragment flanked by Pst I sites. When the amino acid sequence derived from the DNA sequence of pOA Vg 62 was aligned with that of S. gairdneri, X. laevis, C. elegans and D. melanogaster, homologies of 63%, 22%, 21% and 15%, respectively, were obtained. Estradiol induced a vitellogenin gene transcript of 6500 nucleotides at 6 h and reached a maximum at 72 h after stimulation.
Source Title: Biochemistry International
URI: http://scholarbank.nus.edu.sg/handle/10635/106769
ISSN: 01585231
Appears in Collections:Staff Publications

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