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|Title:||Ammonia production and kinetic properties of glutamate dehydrogenase in the sipinculid Phascolosoma arcuatum exposed to anoxia||Authors:||Ip, Y.K.
|Issue Date:||May-1994||Citation:||Ip, Y.K., Peng, K.W., Chew, S.F., Lim, R.W.L., Tan, G.Q. (1994-05). Ammonia production and kinetic properties of glutamate dehydrogenase in the sipinculid Phascolosoma arcuatum exposed to anoxia. Marine Biology 119 (2) : 261-266. ScholarBank@NUS Repository. https://doi.org/10.1007/BF00349565||Abstract:||The amounts of total NH4 +detected in the external media in which Phascolosoma arcuatum had been exposed to various periods of anoxia were significantly greater than those in which the worms were exposed to normoxia for a similar period. The increased NH4 +production by P. arcuatum during anoxic exposure was unlikely to be due to an increased catabolism of adenine nucleotides or urea. In contrast, there were significant decreases in the concentrations of several free amino acids in the coelomic plasma and body tissues of individuals during the 48 h of anoxic exposure. The amount of NH4 +produced by the anoxic P. arcuatum could be accounted for by the decreases in the concentrations of aspartate or glycine. Increases in the catabolism of free amino acids (FAA), leading to the increased production of NH4 +, in P. arcuatum during anoxia were supported by the detection of significant changes in the kinetic properties of glutamate dehydrogenase (GDH), in the deaminating direction, from worms exposed to anoxia for 48 h. The apparent increase in the affinity of GDH from the anoxic worm to glutamate would bring about a greater deaminating activity at physiological concentrations of ths substrate. P. arcuatum used in these experiments were collected from the mangrove swamp at Mandai, Singapore between 1990 and 1993. © 1994 Springer-Verlag.||Source Title:||Marine Biology||URI:||http://scholarbank.nus.edu.sg/handle/10635/106749||ISSN:||00253162||DOI:||10.1007/BF00349565|
|Appears in Collections:||Staff Publications|
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