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https://doi.org/10.1016/j.jchromb.2006.12.056
Title: | Simultaneous determination of irinotecan (CPT-11) and SN-38 in tissue culture media and cancer cells by high performance liquid chromatography: Application to cellular metabolism and accumulation studies | Authors: | Hu, Z.-P. Yang, X.-X. Chen, X. Chan, E. Duan, W. Zhou, S.-F. |
Keywords: | Accumulation CPT-11 HPLC Metabolism SN-38 |
Issue Date: | 1-May-2007 | Citation: | Hu, Z.-P., Yang, X.-X., Chen, X., Chan, E., Duan, W., Zhou, S.-F. (2007-05-01). Simultaneous determination of irinotecan (CPT-11) and SN-38 in tissue culture media and cancer cells by high performance liquid chromatography: Application to cellular metabolism and accumulation studies. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 850 (1-2) : 575-580. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2006.12.056 | Abstract: | A simple and sensitive HPLC method was developed to simultaneously determine CPT-11 and its major metabolite SN-38 in culture media and cell lysates. Camptothecin (CPT) was used as internal standard (I.S.). Compounds were eluted with acetonitrile-50 mM disodium hydrogen phosphate buffer containing 10 mM sodium 1-heptane-sulfonate, with the pH adjusted to 3.0 using 85% (w/v) orthophosphoric acid (27/73, v/v) by a Hyperclon ODS (C18) column (200 mm × 4.6 mm i.d.), with detection at excitation and emission wavelengths of 380 and 540 nm, respectively. The average extraction efficiencies were 96.9-108.3% for CPT-11 in culture media and 94.3-107.2% for CPT-11 in cell lysates; and 87.7-106.8% for SN-38 in culture media and 90.1-105.6% for SN-38 in cell lysates. Within- and between-day precision and accuracy varied from 0.1 to 10.3%. The limit of quantitation (precision and accuracy | Source Title: | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences | URI: | http://scholarbank.nus.edu.sg/handle/10635/106338 | ISSN: | 15700232 | DOI: | 10.1016/j.jchromb.2006.12.056 |
Appears in Collections: | Staff Publications |
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