Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jchromb.2006.12.056
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dc.titleSimultaneous determination of irinotecan (CPT-11) and SN-38 in tissue culture media and cancer cells by high performance liquid chromatography: Application to cellular metabolism and accumulation studies
dc.contributor.authorHu, Z.-P.
dc.contributor.authorYang, X.-X.
dc.contributor.authorChen, X.
dc.contributor.authorChan, E.
dc.contributor.authorDuan, W.
dc.contributor.authorZhou, S.-F.
dc.date.accessioned2014-10-29T01:58:34Z
dc.date.available2014-10-29T01:58:34Z
dc.date.issued2007-05-01
dc.identifier.citationHu, Z.-P., Yang, X.-X., Chen, X., Chan, E., Duan, W., Zhou, S.-F. (2007-05-01). Simultaneous determination of irinotecan (CPT-11) and SN-38 in tissue culture media and cancer cells by high performance liquid chromatography: Application to cellular metabolism and accumulation studies. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 850 (1-2) : 575-580. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2006.12.056
dc.identifier.issn15700232
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/106338
dc.description.abstractA simple and sensitive HPLC method was developed to simultaneously determine CPT-11 and its major metabolite SN-38 in culture media and cell lysates. Camptothecin (CPT) was used as internal standard (I.S.). Compounds were eluted with acetonitrile-50 mM disodium hydrogen phosphate buffer containing 10 mM sodium 1-heptane-sulfonate, with the pH adjusted to 3.0 using 85% (w/v) orthophosphoric acid (27/73, v/v) by a Hyperclon ODS (C18) column (200 mm × 4.6 mm i.d.), with detection at excitation and emission wavelengths of 380 and 540 nm, respectively. The average extraction efficiencies were 96.9-108.3% for CPT-11 in culture media and 94.3-107.2% for CPT-11 in cell lysates; and 87.7-106.8% for SN-38 in culture media and 90.1-105.6% for SN-38 in cell lysates. Within- and between-day precision and accuracy varied from 0.1 to 10.3%. The limit of quantitation (precision and accuracy
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jchromb.2006.12.056
dc.sourceScopus
dc.subjectAccumulation
dc.subjectCPT-11
dc.subjectHPLC
dc.subjectMetabolism
dc.subjectSN-38
dc.typeArticle
dc.contributor.departmentPHARMACY
dc.description.doi10.1016/j.jchromb.2006.12.056
dc.description.sourcetitleJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
dc.description.volume850
dc.description.issue1-2
dc.description.page575-580
dc.description.codenJCBAA
dc.identifier.isiut000246703500077
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