Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jchromb.2006.12.056
Title: Simultaneous determination of irinotecan (CPT-11) and SN-38 in tissue culture media and cancer cells by high performance liquid chromatography: Application to cellular metabolism and accumulation studies
Authors: Hu, Z.-P.
Yang, X.-X.
Chen, X.
Chan, E. 
Duan, W.
Zhou, S.-F. 
Keywords: Accumulation
CPT-11
HPLC
Metabolism
SN-38
Issue Date: 1-May-2007
Citation: Hu, Z.-P., Yang, X.-X., Chen, X., Chan, E., Duan, W., Zhou, S.-F. (2007-05-01). Simultaneous determination of irinotecan (CPT-11) and SN-38 in tissue culture media and cancer cells by high performance liquid chromatography: Application to cellular metabolism and accumulation studies. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 850 (1-2) : 575-580. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2006.12.056
Abstract: A simple and sensitive HPLC method was developed to simultaneously determine CPT-11 and its major metabolite SN-38 in culture media and cell lysates. Camptothecin (CPT) was used as internal standard (I.S.). Compounds were eluted with acetonitrile-50 mM disodium hydrogen phosphate buffer containing 10 mM sodium 1-heptane-sulfonate, with the pH adjusted to 3.0 using 85% (w/v) orthophosphoric acid (27/73, v/v) by a Hyperclon ODS (C18) column (200 mm × 4.6 mm i.d.), with detection at excitation and emission wavelengths of 380 and 540 nm, respectively. The average extraction efficiencies were 96.9-108.3% for CPT-11 in culture media and 94.3-107.2% for CPT-11 in cell lysates; and 87.7-106.8% for SN-38 in culture media and 90.1-105.6% for SN-38 in cell lysates. Within- and between-day precision and accuracy varied from 0.1 to 10.3%. The limit of quantitation (precision and accuracy
Source Title: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
URI: http://scholarbank.nus.edu.sg/handle/10635/106338
ISSN: 15700232
DOI: 10.1016/j.jchromb.2006.12.056
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