Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jchromb.2006.07.062
Title: Simple reversed-phase liquid chromatographic assay for simultaneous quantification of free mycophenolic acid and its glucuronide metabolite in human plasma
Authors: Yau, W.-P. 
Vathsala, A.
Lou, H.-X.
Zhou, S.F. 
Chan, E. 
Keywords: Free drug
Human plasma
Mycophenolic acid
Mycophenolic acid glucuronide
Reversed-phase HPLC
Issue Date: 1-Feb-2007
Citation: Yau, W.-P., Vathsala, A., Lou, H.-X., Zhou, S.F., Chan, E. (2007-02-01). Simple reversed-phase liquid chromatographic assay for simultaneous quantification of free mycophenolic acid and its glucuronide metabolite in human plasma. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 846 (1-2) : 313-318. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2006.07.062
Abstract: A reversed-phase HPLC-UV method, involving simple instrumental setup and mobile phase without ion-pairing reagent, was developed and validated for direct simultaneous quantification of free mycophenolic acid (MPA) and its major metabolite MPA-glucuronide (MPAG) in human plasma. Both free MPA and MPAG were isolated from plasma samples using ultrafiltration prior to analysis. Each chromatographic run was completed within 13 min. The optimized method showed good performance in terms of specificity, linearity (r2 = 0.9999), sensitivity (limit of quantitation (LOQ): 0.005 mg/L for MPA; 1 mg/L for MPAG), and intra- and inter-day precision (R.S.D. < 7%). This assay was successfully applied to free MPA and MPAG measurements in clinical samples. © 2006 Elsevier B.V. All rights reserved.
Source Title: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
URI: http://scholarbank.nus.edu.sg/handle/10635/106334
ISSN: 15700232
DOI: 10.1016/j.jchromb.2006.07.062
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