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|Title:||Sensitive high-performance liquid chromatography/mass spectrometry method for determination of steviol in rat plasma||Authors:||Wang, L.Z.
|Issue Date:||2004||Citation:||Wang, L.Z.,Goh, B.C.,Fan, L.,Lee, H.S. (2004). Sensitive high-performance liquid chromatography/mass spectrometry method for determination of steviol in rat plasma. Rapid Communications in Mass Spectrometry 18 (1) : 83-86. ScholarBank@NUS Repository.||Abstract:||The main toxicological concern of stevioside, a highly potent sweetener from S. rebaudiana, is its main metabolite, steviol. To determine very low levels of steviol in in vivo experiments, a sensitive liquid chromatography/atmospheric pressure chemical ionization mass spectrometry (LC/APCI-MS) method was developed for quantifying steviol in rat plasma after oral administration of a single dose of stevioside (0.5 g/kg). The sample preparation uses liquid-liquid extraction with tert-butyl methyl ether in an acidic environment. The retention time of steviol was 10.5 min. The assay was linear over the range 2-1000 ng/mL with a lower limit of detection of 1 ng/mL. The intra- and inter-day precision were||Source Title:||Rapid Communications in Mass Spectrometry||URI:||http://scholarbank.nus.edu.sg/handle/10635/106327||ISSN:||09514198|
|Appears in Collections:||Staff Publications|
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