Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0731-7085(02)00611-8
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dc.titleQuantitation of paclitaxel in micro-sample rat plasma by a sensitive reversed-phase HPLC assay
dc.contributor.authorWang, L.Z.
dc.contributor.authorHo, P.C.
dc.contributor.authorLee, H.S.
dc.contributor.authorVaddi, H.K.
dc.contributor.authorChan, Y.W.
dc.contributor.authorYung, C.S.
dc.date.accessioned2014-10-29T01:57:47Z
dc.date.available2014-10-29T01:57:47Z
dc.date.issued2003-02-26
dc.identifier.citationWang, L.Z., Ho, P.C., Lee, H.S., Vaddi, H.K., Chan, Y.W., Yung, C.S. (2003-02-26). Quantitation of paclitaxel in micro-sample rat plasma by a sensitive reversed-phase HPLC assay. Journal of Pharmaceutical and Biomedical Analysis 31 (2) : 283-289. ScholarBank@NUS Repository. https://doi.org/10.1016/S0731-7085(02)00611-8
dc.identifier.issn07317085
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/106282
dc.description.abstractA sensitive high-performance liquid chromatographic (HPLC) method was developed for the determination of paclitaxel in micro-samples of rat plasma in order to study the mechanism of enhanced systemic exposure of paclitaxel co-administered with P-glycoprotein inhibitors. The assay involved solid-phase extraction procedures using 2′-methylpaclitaxel as the internal standard. Chromatographic separations were achieved using a ZORBAX ODS C18 column and mobile phase consisting of acetonitrile, methanol and ammonium acetate buffer (10 mM, pH 5.0) (48.5:16.5:35) pumped at 0.8 ml/min. The effluents were measured for UV absorption at 227 nm, with retention times of 8.5 and 11.0 min for paclitaxel and 2′-methylpaclitaxel, respectively. The chromatographic separation was excellent, with no endogenous interference. The standard curves showed a good linearity (r=0.9994) over the concentration ranges of 10-1000 ng/ml. At 1000 ng/ml, the absolute recoveries of paclitaxel and 2′-methylpaclitaxel are 89 and 90%, respectively. The intra- and inter-day variabilities of paclitaxel were both less than 15%. This validated method for the assay of paclitaxel in micro-sample rat plasma made it feasible to study the pharmacokinetics of the drug in a single rat. © 2002 Elsevier Science B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0731-7085(02)00611-8
dc.sourceScopus
dc.subjectPaclitaxel
dc.subjectRat plasma
dc.subjectReversed-phase HPLC
dc.typeArticle
dc.contributor.departmentPHARMACY
dc.description.doi10.1016/S0731-7085(02)00611-8
dc.description.sourcetitleJournal of Pharmaceutical and Biomedical Analysis
dc.description.volume31
dc.description.issue2
dc.description.page283-289
dc.description.codenJPBAD
dc.identifier.isiut000181347700007
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