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Title: Quantitation of paclitaxel in micro-sample rat plasma by a sensitive reversed-phase HPLC assay
Authors: Wang, L.Z. 
Ho, P.C. 
Lee, H.S.
Vaddi, H.K.
Chan, Y.W.
Yung, C.S. 
Keywords: Paclitaxel
Rat plasma
Reversed-phase HPLC
Issue Date: 26-Feb-2003
Citation: Wang, L.Z., Ho, P.C., Lee, H.S., Vaddi, H.K., Chan, Y.W., Yung, C.S. (2003-02-26). Quantitation of paclitaxel in micro-sample rat plasma by a sensitive reversed-phase HPLC assay. Journal of Pharmaceutical and Biomedical Analysis 31 (2) : 283-289. ScholarBank@NUS Repository.
Abstract: A sensitive high-performance liquid chromatographic (HPLC) method was developed for the determination of paclitaxel in micro-samples of rat plasma in order to study the mechanism of enhanced systemic exposure of paclitaxel co-administered with P-glycoprotein inhibitors. The assay involved solid-phase extraction procedures using 2′-methylpaclitaxel as the internal standard. Chromatographic separations were achieved using a ZORBAX ODS C18 column and mobile phase consisting of acetonitrile, methanol and ammonium acetate buffer (10 mM, pH 5.0) (48.5:16.5:35) pumped at 0.8 ml/min. The effluents were measured for UV absorption at 227 nm, with retention times of 8.5 and 11.0 min for paclitaxel and 2′-methylpaclitaxel, respectively. The chromatographic separation was excellent, with no endogenous interference. The standard curves showed a good linearity (r=0.9994) over the concentration ranges of 10-1000 ng/ml. At 1000 ng/ml, the absolute recoveries of paclitaxel and 2′-methylpaclitaxel are 89 and 90%, respectively. The intra- and inter-day variabilities of paclitaxel were both less than 15%. This validated method for the assay of paclitaxel in micro-sample rat plasma made it feasible to study the pharmacokinetics of the drug in a single rat. © 2002 Elsevier Science B.V. All rights reserved.
Source Title: Journal of Pharmaceutical and Biomedical Analysis
ISSN: 07317085
DOI: 10.1016/S0731-7085(02)00611-8
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