Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jchromb.2013.05.023
Title: Quantification of pinosylvin in rat plasma by liquid chromatography-tandem mass spectrometry: Application to a pre-clinical pharmacokinetic study
Authors: Yeo, S.C.M.
Luo, W.
Wu, J.
Ho, P.C. 
Lin, H.-S. 
Keywords: LC-ESI-MS/MS
Oral bioavailability
Pharmacokinetics
Pinosylvin
Resveratrol
Issue Date: 5-Jul-2013
Citation: Yeo, S.C.M., Luo, W., Wu, J., Ho, P.C., Lin, H.-S. (2013-07-05). Quantification of pinosylvin in rat plasma by liquid chromatography-tandem mass spectrometry: Application to a pre-clinical pharmacokinetic study. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences 931 : 68-74. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jchromb.2013.05.023
Abstract: Pinosylvin (trans-3,5-dihydroxystilbene), a naturally occurring analogue of resveratrol (trans-3,5,4'-trihydoxystilbene), exhibited various beneficial pharmacological activities in pre-clinical studies. To further probe its potential medicinal application, a sensitive liquid chromatography-tandem mass spectrometry method (LC-MS/MS) was developed and validated for the quantification of pinosylvin in rat plasma. A simple protein precipitation procedure was used for plasma cleanup before analysis by LC-MS/MS with electrospray ionisation and multiple reaction monitoring in its negative ion mode. This LC-MS/MS method demonstrated good selectivity, accuracy (intra- and inter-day analytical recovery within 100±7.7%), precision (intra- and inter-day coefficient of variation0.99) over the range of 1-1000ng/mL. The pharmacokinetic profiles of pinosylvin were subsequently assessed in Sprague-Dawley rats. Following intravenous administration (5 or 10mg/kg), plasma levels of pinosylvin declined rapidly with a short half-life (t1/2
Source Title: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
URI: http://scholarbank.nus.edu.sg/handle/10635/106276
ISSN: 15700232
DOI: 10.1016/j.jchromb.2013.05.023
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