Please use this identifier to cite or link to this item:
https://doi.org/10.1124/dmd.107.019737
DC Field | Value | |
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dc.title | Effects of spice constituents on P-glycoprotein-mediated transport and CYP3A4-mediated metabolism in vitro | |
dc.contributor.author | Zhang, W. | |
dc.contributor.author | Lim, L.-Y. | |
dc.date.accessioned | 2014-10-29T01:52:16Z | |
dc.date.available | 2014-10-29T01:52:16Z | |
dc.date.issued | 2008-07 | |
dc.identifier.citation | Zhang, W., Lim, L.-Y. (2008-07). Effects of spice constituents on P-glycoprotein-mediated transport and CYP3A4-mediated metabolism in vitro. Drug Metabolism and Disposition 36 (7) : 1283-1290. ScholarBank@NUS Repository. https://doi.org/10.1124/dmd.107.019737 | |
dc.identifier.issn | 00909556 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/105906 | |
dc.description.abstract | The effects of eight components from six commonly consumed spices on P-glycoprotein (P-gp) transport and CYP3A4 metabolism were evaluated in vitro. P-gp-mediated [3H]digoxin fluxes across the L-MDR1 (LLC-PK1 cells transfected with human MDR1 gene) and Caco-2 (human colon carcinoma) cell monolayers showed a marked asymmetry compared with that in the LLC-PK1 (porcine kidney epithelial cells) cell monolayers. Curcumin (from turmeric) at 30 to 60 μM and 6-gingerol (from ginger) at 100 to 500 μM were observed to inhibit P-gp-mediated [3H]digoxin transport in L-MDR1 and Caco-2 cells. Effects of spices on midazolam (MDZ) 1′-hydroxylation and 4-hydroxylation of CYP3A4 activity were determined in pooled human liver microsomes (HLM). The following IC50 values for effects of spices on MDZ 1′-hydroxylation in HLM were obtained: 29 μM for curcumin, 1.17 mM for allyl methyl disulfide (AMD) (from Chinese chive), 1.02 mM for 1,8-cineole (from coriander), and 1.28 mM for β-caryophyllene (from curry leaf). CYP3A4-mediated 4-hydroxylation of MDZ was inhibited by curcumin at 30, 45, and 60 μM (4-hydroxy-MDZ formation was decreased to 52, 30, and 29%, respectively, compared with control), by 6-gingerol at 60, 100, and 500 μM (71, 68, and 38%), by AMD at 1 and 4 mM (29 and 14%), by d-limonene (from coriander) at 4 mM (65%), by 1,8-cineole at 0.5, 1, and 4 mM (74, 64, and 59%), and by citral (from lemon-grass) at 1 mM (59%). Among the spices that showed inhibitory effect on MDZ metabolism in HLM, only AMD showed a preincu-bation time-dependent inhibitory effect on MDZ metabolism in HLM, suggesting the AMD as an irreversible CYP3A4 inhibitor. Copyright © 2008 by The American Society for Pharmacology and Experimental Therapeutics. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1124/dmd.107.019737 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | PHARMACY | |
dc.description.doi | 10.1124/dmd.107.019737 | |
dc.description.sourcetitle | Drug Metabolism and Disposition | |
dc.description.volume | 36 | |
dc.description.issue | 7 | |
dc.description.page | 1283-1290 | |
dc.description.coden | DMDSA | |
dc.identifier.isiut | 000256905100016 | |
Appears in Collections: | Staff Publications |
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