Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/105637
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dc.titleAn expedient assay for determination of gemcitabine and its metabolite in human plasma using isocratic ion-pair reversed-phase high-performance liquid chromatography
dc.contributor.authorWang, L.-Z.
dc.contributor.authorGoh, B.-C.
dc.contributor.authorLee, H.-S.
dc.contributor.authorNoordhuis, P.
dc.contributor.authorPeters, G.J.
dc.date.accessioned2014-10-29T01:48:24Z
dc.date.available2014-10-29T01:48:24Z
dc.date.issued2003-10
dc.identifier.citationWang, L.-Z., Goh, B.-C., Lee, H.-S., Noordhuis, P., Peters, G.J. (2003-10). An expedient assay for determination of gemcitabine and its metabolite in human plasma using isocratic ion-pair reversed-phase high-performance liquid chromatography. Therapeutic Drug Monitoring 25 (5) : 552-557. ScholarBank@NUS Repository.
dc.identifier.issn01634356
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/105637
dc.description.abstractAn expedient method is presented for determination in human plasma of gemcitabine and its metabolite 2′,2′-difluorodeoxyuridine (dFdU) by ion-pair reversed-phase HPLC. Samples were simply prepared by protein precipitation. Separation was processed on a Thermo Hypersil column (250 × 4.6 mm, 5 μm Hypersil® BDS C18) with UV detection at 272 nm. The mobile phase consisted of 17% methanol and 83% phosphate buffer (20 mM, pH 3.1) containing 10 mM sodium 1-heptanesulfonate with a flow rate of 0.8 mL/min. The lower limit of quantification (LLOQ) of gemcitabine was 0.08 μg/mL with linear response over the range 0.08-20.0 μg/mL, and LLOQ of dFdU was 0.1 μg/mL with linear response over the range 0.1-50.0 μg/mL. Assay accuracy for both compounds was within ± 4%. The coefficient of variation (CV %) for intra- and interday precision for both compounds was
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1097/00007691-200310000-00003
dc.sourceScopus
dc.subject2′,2′-difluorodeoxyuridine
dc.subjectGemcitabine
dc.subjectHPLC
dc.subjectIon-pair
dc.subjectPlasma
dc.typeArticle
dc.contributor.departmentPHARMACY
dc.description.sourcetitleTherapeutic Drug Monitoring
dc.description.volume25
dc.description.issue5
dc.description.page552-557
dc.description.codenTDMOD
dc.identifier.isiut000185579300003
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