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Title: A simple assay for determination of levetiracetam in rat dried blood spots by LC-MS/MS
Authors: Luo, W.
Kong, S.T.
Yang, S.
Chan, B.C.W.
Ho, P.C. 
Issue Date: Aug-2013
Citation: Luo, W., Kong, S.T., Yang, S., Chan, B.C.W., Ho, P.C. (2013-08). A simple assay for determination of levetiracetam in rat dried blood spots by LC-MS/MS. Bioanalysis 5 (15) : 1843-1851. ScholarBank@NUS Repository.
Abstract: Background: A simple LC-MS/MS method was developed and validated for the quantification of levetiracetam (LEV, Keppra®), a broad-spectrum antiepileptic drug (AED) in rat dried blood spots (DBS). LEV was simply extracted with methanol spiked with adenosine (ADE) as IS before LC-MS/MS analysis. The correlation between the DBS and plasma concentrations of LEV was also determined. Results: Linearity was from 0.067-60 μg/ml for LEV in DBS samples. The intra- and inter-day accuracy and precision of the assay met validation acceptance criteria. The developed assay was applied to monitor levetiracetam DBS levels in Sprague-Dawley rats after intravenous administration. DBS concentrations were well correlated to the plasma concentrations (R2 = 0.9399), as fraction of LEV bound to blood cells remains very constant (0.466 ± 0.041) over a wide concentration range. Conclusion: The study illustrated that DBS could be used as alternative matrix for monitoring LEV in preclinical studies. © 2013 Future Science Ltd.
Source Title: Bioanalysis
ISSN: 17576180
DOI: 10.4155/bio.13.143
Appears in Collections:Staff Publications

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