Please use this identifier to cite or link to this item:
|Title:||A simple and sensitive HPLC-UV method for the quantification of piceatannol analog trans-3,5,3′,4′-tetramethoxystilbene in rat plasma and its application for a pre-clinical pharmacokinetic study||Authors:||Lin, H.-S.
|Issue Date:||5-Feb-2010||Citation:||Lin, H.-S., Tringali, C., Spatafora, C., Wu, C., Ho, P.C. (2010-02-05). A simple and sensitive HPLC-UV method for the quantification of piceatannol analog trans-3,5,3′,4′-tetramethoxystilbene in rat plasma and its application for a pre-clinical pharmacokinetic study. Journal of Pharmaceutical and Biomedical Analysis 51 (3) : 679-684. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jpba.2009.09.024||Abstract:||A simple and sensitive HPLC-UV method was developed and validated for the quantification of piceatannol analog trans-3,5,3′,4′-tetramethoxystilbene (M-PIC) in rat plasma. Following protein precipitation with three volumes of acetonitrile, the analytes were separated on a RP-HPLC column, which was protected by a guard column through gradient delivery of a mixture of acetonitrile-water at 40 °C. The UV absorbance at 325 nm was recorded to quantify M-PIC. The retention time of M-PIC and trans-3,5-dimethoxystilbene (internal standard) was 7.4 and 8.4 min, respectively. The calibration curves were linear (R2 > 0.9989) with a lower limit of quantification of 15 ng/ml. The intra- and inter-day precisions, in terms of RSD, were all lower than 7.5%. The average analytical recovery ranged from 97.0 to 104.3% while the average absolute recovery ranged from 101.8 to 105.0%. This reliable HPLC method was subsequently applied to assess the pharmacokinetic profile of M-PIC in Sprague-Dawley rats using 2-hydroxypropyl-β-cyclodextrin as a dosing vehicle. The terminal elimination half-life (t1/2λz) and clearance (Cl) of M-PIC were 313 ± 20 min and 33.1 ± 3.9 ml/min/kg, respectively; and its absolute oral bioavailability was as high as 50.7 ± 15.0%. M-PIC appeared to have a favorable pharmacokinetic profile and further pharmacological investigation on this phyto-stilbene was warranted. © 2009 Elsevier B.V. All rights reserved.||Source Title:||Journal of Pharmaceutical and Biomedical Analysis||URI:||http://scholarbank.nus.edu.sg/handle/10635/105593||ISSN:||07317085||DOI:||10.1016/j.jpba.2009.09.024|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 18, 2019
WEB OF SCIENCETM
checked on Oct 18, 2019
checked on Oct 11, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.