Please use this identifier to cite or link to this item:
|Title:||Testing linkage disequilibrium from pooled dna: A contingency table perspective||Authors:||Yang, Y.
|Issue Date:||Dec-2008||Citation:||Yang, Y., Xu, J., Ying, Z., Ott, J. (2008-12). Testing linkage disequilibrium from pooled dna: A contingency table perspective. Statistics in Medicine 27 (28) : 5801-5815. ScholarBank@NUS Repository. https://doi.org/10.1002/sim.3407||Abstract:||Pooling DNA samples of multiple individuals has been advocated as a method to reduce genotyping costs. Under such a scheme, only the allele counts at each locus, not the haplotype information, are bserved. We develop a systematic way for handling such data by formulating the problem in terms ofcontingency tables, where pooled allele counts are expressed as the margins and the haplotype counts correspond to the unobserved cell counts. We show that the cell frequencies can be uniquely determined from the marginal frequencies under the usual Hardy-Weinberg equilibrium (HWE) assumption and thatthe maximum likelihood estimates of haplotype frequencies are consistent and asymptotically normal as the number of pools increases. The limiting covariance matrix is shown to be closely related to the extended hypergeometric distribution. Our results are used to derive Wald-type tests for linkage disequilibrium (LD) coefficient using pooled data. It is discovered that pooling is not efficient in testing weak LD despiteits efficiency in estimating haplotype frequencies. We also show by simulations that the proposed LD tests are robust to slight deviation from HWE and to minor genotype error. Applications to two real angiotensinogen gene data sets are also provided. © 2008 John Wiley & Sons, Ltd.||Source Title:||Statistics in Medicine||URI:||http://scholarbank.nus.edu.sg/handle/10635/105403||ISSN:||02776715||DOI:||10.1002/sim.3407|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 18, 2022
WEB OF SCIENCETM
checked on Jan 11, 2022
checked on Jan 20, 2022
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.