Please use this identifier to cite or link to this item: https://doi.org/10.1007/s00299-007-0460-2
Title: Virus-induced gene silencing for functional analysis of selected genes
Authors: Godge, M.R. 
Purkayastha, A.
Dasgupta, I.
Kumar, P.P. 
Keywords: Functional genomics
Gene silencing
VIGS
Viral vectors
Issue Date: Feb-2008
Citation: Godge, M.R., Purkayastha, A., Dasgupta, I., Kumar, P.P. (2008-02). Virus-induced gene silencing for functional analysis of selected genes. Plant Cell Reports 27 (2) : 209-219. ScholarBank@NUS Repository. https://doi.org/10.1007/s00299-007-0460-2
Abstract: Virus-induced gene silencing (VIGS) is a technology that exploits an RNA-mediated antiviral defense mechanism and has been shown to be of great potential in plant reverse genetics. Circumvention of plant transformation, methodological simplicity, robustness, and speedy results makes VIGS an attractive alternative instrument in functional genomics, even in a high throughput fashion. The system is well established in Nicotiana benthamiana, and efforts are being made to improve VIGS in other species, including monocots. Here, we discuss the issues specific to the application of VIGS technology to determine gene function, which has revealed the roles of a variety of genes in disease resistance, abiotic stress, cellular signaling and secondary metabolite biosynthesis. © 2007 Springer-Verlag.
Source Title: Plant Cell Reports
URI: http://scholarbank.nus.edu.sg/handle/10635/102585
ISSN: 07217714
DOI: 10.1007/s00299-007-0460-2
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