Please use this identifier to cite or link to this item: https://doi.org/10.1080/10715760410001694008
Title: Do mitochondria make nitric oxide? No?
Authors: Tay, Y.M.S.
Lim, K.S.
Sheu, F.-S. 
Jenner, A.
Whiteman, M.
Wong, K.P.
Halliwell, B.
Keywords: Artifact
Griess assay
Mitochondria
Nitric oxide
Nitric oxide synthase
Issue Date: Jun-2004
Citation: Tay, Y.M.S., Lim, K.S., Sheu, F.-S., Jenner, A., Whiteman, M., Wong, K.P., Halliwell, B. (2004-06). Do mitochondria make nitric oxide? No?. Free Radical Research 38 (6) : 591-599. ScholarBank@NUS Repository. https://doi.org/10.1080/10715760410001694008
Abstract: Several papers have claimed that mitochondria contain nitric oxide synthase (NOS) and make nitric oxide (NO.) in amounts sufficient to affect mitochondrial respiration. However, we found that the addition of L-arginine or the NOS inhibitor L-NMMA to intact rat liver mitochondria did not have any effect on the respiratory rate in both State 3 and State 4. We did not detect mitochondrial NO. production by the oxymyoglobin oxidation assay, or electrochemically using an NO. electrode. An apparent NO. production detected by the Griess assay was identified as an artifact. NO. generated by eNOS added to the mitochondria could easily be detected, although succinate-supplemented mitochondria appeared to consume NO.. Our data show that NO. production by normal rat liver mitochondria cannot be detected in our laboratory, even though the levels of production claimed in the literature should easily have been measured by the techniques used. The implications for the putative mitochondrial NOS are discussed. © 2004 Taylor & Francis Ltd.
Source Title: Free Radical Research
URI: http://scholarbank.nus.edu.sg/handle/10635/102435
ISSN: 10715762
DOI: 10.1080/10715760410001694008
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