Please use this identifier to cite or link to this item: https://doi.org/10.1002/9781118610749.ch20
Title: Delivery of Micro RNA Sponges for Interrogation of Micro RNA Function In Vitro and In Vivo
Authors: Lin, J.
Wang, S. 
Keywords: Cell-specific promoters
Combinatorial RNAi
Constitutive promoters
Inducible promoters
MiRNA
Issue Date: 26-Apr-2013
Citation: Lin, J.,Wang, S. (2013-04-26). Delivery of Micro RNA Sponges for Interrogation of Micro RNA Function In Vitro and In Vivo. Advanced Delivery and Therapeutic Applications of RNAi : 483-504. ScholarBank@NUS Repository. https://doi.org/10.1002/9781118610749.ch20
Abstract: Promoters are regulatory elements that provide precise control of magnitude, specificity and duration of shRNA expression and gene silencing. The selection of a suitable promoter is therefore critical in the design of a shRNA expression system. We discuss the use of different promoters for shRNA expression, including constitutive promoters, inducible promoters, and tissue/tumor specific promoters. Recently, miRNA-based shRNAs have received increasing attention as a promising RNAi approach. We have therefore also summarized the use of miRNA backbone to enhance gene silencing and to reduce toxicities. The application of miRNA-shRNA for combinatorial gene therapy is also discussed. © 2013 by John Wiley and Sons, Ltd.
Source Title: Advanced Delivery and Therapeutic Applications of RNAi
URI: http://scholarbank.nus.edu.sg/handle/10635/102306
ISBN: 9781119976868
DOI: 10.1002/9781118610749.ch20
Appears in Collections:Staff Publications

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