Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/102201
DC FieldValue
dc.titleComparison of coat protein genes of Cymbidium mosaic virus and Odontoglossum ringspot virus from Phalaenopsis and Cymbidium in Korea
dc.contributor.authorChoi, S.H.
dc.contributor.authorRyu, K.H.
dc.contributor.authorWong, S.M.
dc.date.accessioned2014-10-27T08:45:39Z
dc.date.available2014-10-27T08:45:39Z
dc.date.issued2006-10
dc.identifier.citationChoi, S.H.,Ryu, K.H.,Wong, S.M. (2006-10). Comparison of coat protein genes of Cymbidium mosaic virus and Odontoglossum ringspot virus from Phalaenopsis and Cymbidium in Korea. Acta Horticulturae 722 : 293-298. ScholarBank@NUS Repository.
dc.identifier.isbn9066056495
dc.identifier.issn05677572
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/102201
dc.description.abstractOf more than 50 orchid viruses, the potexvirus Cymbidum mosaic virus (CymMV) and the tobamovirus Odontoglossum ringspot virus (ORSV) are the two most prevalent and economically important viruses that have attained a worldwide distribution. To detect possible molecular heterogeneity, we determined the coat protein (CP) gene sequences of CymMV and ORSV infecting different genera of orchid from geographically distinct isolates. The variabilities in the coat protein gene (CP) sequences of CymMV and ORSV that infect Phalaenopsis and Cymbidium orchids were investigated. Infected leaf samples of Phalaenopsis and Cymbidium were screened by RT-PCR with specific primers to both viruses. RT-PCR products of the viruses were cloned and their nucleotide sequences were determined. All determined CymMV CP genes have the same size of amino acid sequences (216 a.a), except for one clone derived from Cymbidium in Korea (197 a.a). The sequence data were compared with available published coat protein gene sequences of CymMV and ORSV, including those from Korea, Singapore, Japan and Thailand. Multiple alignments of CP genes of the viruses revealed that over 66.4% and 91.8% amino acid sequence identities with CymMV and ORSV, respectively, were observed. Such high sequence conservation suggests that both CymMV and ORSV CP genes are suitable candidates to provide resistance in orchids cultivated in different geographical locations. CPmediated resistance has been a widely used pathogenderived resistance strategy and its success in producing a broad ranging virus resistant orchid would depend on the sequence similarity between the transgenes and the virus infecting the plant.
dc.sourceScopus
dc.subjectCoat protein
dc.subjectCymbidium
dc.subjectCymbidium mosaic virus
dc.subjectOdontoglossum ringspot virus
dc.subjectOrchid virus
dc.subjectPhalaenopsis
dc.subjectRT-PCR
dc.typeConference Paper
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.sourcetitleActa Horticulturae
dc.description.volume722
dc.description.page293-298
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Page view(s)

80
checked on Dec 2, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.