Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/102199
DC FieldValue
dc.titleCarbon nanotube-based labels for sensitive nucleic acids detection
dc.contributor.authorLee, A.C.
dc.contributor.authorYe, J.S.
dc.contributor.authorTan, S.N.
dc.contributor.authorPoenar, D.
dc.contributor.authorSheu, F.S.
dc.contributor.authorHeng, C.K.
dc.contributor.authorLim, T.M.
dc.date.accessioned2014-10-27T08:45:38Z
dc.date.available2014-10-27T08:45:38Z
dc.date.issued2006
dc.identifier.citationLee, A.C.,Ye, J.S.,Tan, S.N.,Poenar, D.,Sheu, F.S.,Heng, C.K.,Lim, T.M. (2006). Carbon nanotube-based labels for sensitive nucleic acids detection. 2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings 2 : 232-235. ScholarBank@NUS Repository.
dc.identifier.isbn0976798573
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/102199
dc.description.abstractA novel multiple-labelled conjugate with carbon nanotubes (CNTs) to amplify hybridization signal was developed to improve the nucleic acids assay sensitivity. The carboxylated CNTs were functionalized by horseradish peroxidase (HRP) and single-stranded oligonucleotides via diimide-activated amidation. Oligonucleotides with specific sequence from SUP-B15 cell line that serves as molecular signatures of human acute lymphocytic leukemia (ALL) was synthesized and used as targets. Results show that both HRP and oligonucleotides were conjugated to the CNTs. The resulting conjugates can hybridize to its complementary targets and function as a label for nucleic acid quantification. These labels are also able to amplify the signals generated by the colorimetric method that significantly enhance the detection limit of the target by at least 1000 folds compared with conventional labels. These labels offer a unique advantage. Visual inspection of target detection was made possible due to the formation of visible aggregates.
dc.sourceScopus
dc.subjectCarbon nanotube
dc.subjectColorimetric
dc.subjectDisease diagnosis
dc.subjectMultiple-label
dc.subjectNucleic acid detection
dc.typeConference Paper
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.sourcetitle2006 NSTI Nanotechnology Conference and Trade Show - NSTI Nanotech 2006 Technical Proceedings
dc.description.volume2
dc.description.page232-235
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Page view(s)

72
checked on Aug 27, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.