Please use this identifier to cite or link to this item: https://doi.org/10.1091/mbc.E06-07-0624
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dc.titleZic3 is required for maintenance of pluripotency in embryonic stem cells
dc.contributor.authorLim, L.S.
dc.contributor.authorLoh, Y.-H.
dc.contributor.authorZhang, W.
dc.contributor.authorLi, Y.
dc.contributor.authorChen, X.
dc.contributor.authorWang, Y.
dc.contributor.authorBakre, M.
dc.contributor.authorNg, H.-H.
dc.contributor.authorStanton, L.W.
dc.date.accessioned2014-10-27T08:45:31Z
dc.date.available2014-10-27T08:45:31Z
dc.date.issued2007-04
dc.identifier.citationLim, L.S., Loh, Y.-H., Zhang, W., Li, Y., Chen, X., Wang, Y., Bakre, M., Ng, H.-H., Stanton, L.W. (2007-04). Zic3 is required for maintenance of pluripotency in embryonic stem cells. Molecular Biology of the Cell 18 (4) : 1348-1358. ScholarBank@NUS Repository. https://doi.org/10.1091/mbc.E06-07-0624
dc.identifier.issn10591524
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/102189
dc.description.abstractEmbryonic stem (ES) cell pluripotency is dependent upon sustained expression of the key transcriptional regulators Oct4, Nanog, and Sox2. Dissection of the regulatory networks downstream of these transcription factors has provided critical insight into the molecular mechanisms that regulate ES cell pluripotency and early differentiation. Here we describe a role for Zic3, a member of the Gli family of zinc finger transcription factors, in the maintenance of pluripotency in ES cells. We show that Zic3 is expressed in ES cells and that this expression is repressed upon differentiation. The expression of Zic3 in pluripotent ES cells is also directly regulated by Oct4, Sox2, and Nanog. Targeted repression of Zic3 in human and mouse ES cells by RNA interference-induced expression of several markers of the endodermal lineage. Notably, the expression of Nanog, a key pluripotency regulator and repressor of extraembryonic endoderm specification in ES cells, was significantly reduced in Zic3 knockdown cells. This suggests that Zic3 may prevent endodermal marker expression through Nanog-regulated pathways. Thus our results extend the ES cell transcriptional network beyond Oct4, Nanog, and Sox2, and further establish that Zic3 plays an important role in the maintenance of pluripotency by preventing endodermal lineage specification in embryonic stem cells. © 2007 by The American Society for Cell Biology.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1091/mbc.E06-07-0624
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1091/mbc.E06-07-0624
dc.description.sourcetitleMolecular Biology of the Cell
dc.description.volume18
dc.description.issue4
dc.description.page1348-1358
dc.description.codenMBCEE
dc.identifier.isiut000245443100020
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