Please use this identifier to cite or link to this item: https://doi.org/10.1128/AEM.69.10.5941-5949.2003
DC FieldValue
dc.titleUtilization of Acyl-Homoserine Lactone Quorum Signals for Growth by a Soil Pseudomonad and Pseudomonas aeruginosa PAO1
dc.contributor.authorHuang, J.J.
dc.contributor.authorHan, J.-I.
dc.contributor.authorZhang, L.-H.
dc.contributor.authorLeadbetter, J.R.
dc.date.accessioned2014-10-27T08:44:54Z
dc.date.available2014-10-27T08:44:54Z
dc.date.issued2003-10
dc.identifier.citationHuang, J.J., Han, J.-I., Zhang, L.-H., Leadbetter, J.R. (2003-10). Utilization of Acyl-Homoserine Lactone Quorum Signals for Growth by a Soil Pseudomonad and Pseudomonas aeruginosa PAO1. Applied and Environmental Microbiology 69 (10) : 5941-5949. ScholarBank@NUS Repository. https://doi.org/10.1128/AEM.69.10.5941-5949.2003
dc.identifier.issn00992240
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/102132
dc.description.abstractAcyl-homoserine lactones (AHLs) are employed by several Proteobacteria as quorum-sensing signals. Past studies have established that these compounds are subject to biochemical decay and can be used as growth nutrients. Here we describe the isolation of a soil bacterium, Pseudomonas strain PAI-A, that degrades 3-oxododecanoyl-homoserine lactone (3OC12HSL) and other long-acyl, but not short-acyl, AHLs as sole energy sources for growth. The small-subunit rRNA gene from strain PAI-A was 98.4% identical to that of Pseudomonas aeruginosa, but the soil isolate did not produce obvious pigments or AHLs or grow under denitrifying conditions or at 42°C. The quorum-sensing bacterium P. aeruginosa, which produces both 3OC12HSL and C4HSL, was examined for the ability to utilize AHLs for growth. It did so with a specificity similar to that of strain PAI-A, i.e., degrading long-acyl but not short-acyl AHLs. In contrast to the growth observed with strain PAI-A, P. aeruginosa strain PAO1 growth on AHLs commenced only after extremely long lag phases. Liquid-chromatography-atmospheric pressure chemical ionization-mass spectrometry analyses indicate that strain PAO1 degrades long-acyl AHLs via an AHL acylase and a homoserine-generating HSL lactonase. A P. aeruginosa gene, pvdQ (PA2385), has previously been identified as being a homologue of the AHL acylase described as occurring in a Ralstonia species. Escherichia coli expressing pvdQ catalyzed the rapid inactivation of long-acyl AHLs and the release of HSL. P. aeruginosa engineered to constitutively express pvdQ did not accumulate its 3OC12HSL quorum signal when grown in rich media. However, pvdQ knockout mutants of P. aeruginosa were still able to grow by utilizing 3OC12HSL. To our knowledge, this is the first report of the degradation of AHLs by pseudomonads or other γ-Proteobacteria, of AHL acylase activity in a quorum-sensing bacterium, of HSL lactonase activity in any bacterium, and of AHL degradation with specificity only towards AHLs with long side chains.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1128/AEM.69.10.5941-5949.2003
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1128/AEM.69.10.5941-5949.2003
dc.description.sourcetitleApplied and Environmental Microbiology
dc.description.volume69
dc.description.issue10
dc.description.page5941-5949
dc.description.codenAEMID
dc.identifier.isiut000185881300028
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

248
checked on Jul 3, 2020

WEB OF SCIENCETM
Citations

223
checked on Jun 25, 2020

Page view(s)

61
checked on Jun 27, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.