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Title: Up-regulation of cytokines in glomerulonephritis associated with murine malaria infection
Authors: Sinniah, R.
Rui-Mei, L.
Kara, A. 
Keywords: Cytokines
Issue Date: 1999
Citation: Sinniah, R., Rui-Mei, L., Kara, A. (1999). Up-regulation of cytokines in glomerulonephritis associated with murine malaria infection. International Journal of Experimental Pathology 80 (2) : 87-95. ScholarBank@NUS Repository.
Abstract: Malaria infections often cause glomerulonephritis (GN), and multiple factors have been implicated in the pathogenesis of glomerular injury. The role of cytokines in malaria associated glomerulonephritis has not been clearly defined. To study the importance of cytokines in malarial nephritis, we investigated the expression of tumour necrosis factor-alpha (TNF-α), interleukin-1-alpha (IL-1α), IL-6, IL-10 and granulocyte macrophagecolony stimulating factor (GM-CSF) in kidneys acutely infected with murine malaria parasite Plasmodium berghei ANKA in C57BL/6J mice. Groups of six mice sacrificed on days 5, 8-10, 15, and 20 postinfection, and normal controls were used for cytokine analysis. Elevated levels of messenger RNA (mRNA) specific for these cytokines in infected kidneys after day 5 postinfection were demonstrated by reverse transcription-polymerase chain reaction (RT- PCR) analysis. Kidney sections stained with specific antibodies against TNF- α, IL-1α, IL-6, IL-10 and GM-CSF by immunohistochemistry showed that the staining for these cytokines on the glomeruli was positive from day 10 postinfection, and increased progressively, mainly in the infiltrating macrophages and the glomerular mesangium. Strong correlation was found between the expression of TNF-α with IL-6, and IL-1α with IL-6. The expression of TNF-α, IL-1α, IL-6, and IL-10 also strongly correlated with the severity of proteinuria. Our findings show that there is up-regulation of cytokines in the pathogenesis of glomerulonephritis associated with murine malaria infection.
Source Title: International Journal of Experimental Pathology
ISSN: 09599673
DOI: 10.1046/j.1365-2613.1999.00101.x
Appears in Collections:Staff Publications

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