Please use this identifier to cite or link to this item: https://doi.org/10.1111/j.1600-0854.2008.00873.x
DC FieldValue
dc.titleUltrastructural study of Golgi duplication in Trypanosoma brucei
dc.contributor.authorYelinek, J.T.
dc.contributor.authorHe, C.Y.
dc.contributor.authorWarren, G.
dc.date.accessioned2014-10-27T08:44:37Z
dc.date.available2014-10-27T08:44:37Z
dc.date.issued2009
dc.identifier.citationYelinek, J.T., He, C.Y., Warren, G. (2009). Ultrastructural study of Golgi duplication in Trypanosoma brucei. Traffic 10 (3) : 300-306. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1600-0854.2008.00873.x
dc.identifier.issn13989219
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/102107
dc.description.abstractGolgi duplication in the protozoan parasite Trypanosoma brucei has been tracked using serial thin section three-dimensional reconstructions of transmission electron micrographs. The old Golgi maintains a constant size (∼0.060 μm3) throughout the cell cycle. A morphologically identifiable new Golgi appears at ∼0.20 of the cell cycle (defined by the size of the nucleus and lasting about 9 h) and grows from ∼0.018 μm3 until it is the same size as the old Golgi (by ∼0.55 of the cell cycle). Morphologically identifiable late Golgi appear at ∼0.58 of the cell cycle, but their volume (∼0.036 μm3) did not change significantly. Cryoimmunoelectron microscopy was used to identify candidates for the earliest new Golgi structures, and these comprised clusters of vesicles containing Golgi reassembly stacking protein (GRASP) near an endoplasmic reticulum exit site. These results, combined with earlier fluorescence data, suggest that the new Golgi begins functioning before cisternal stacks are formed. © 2009 The Authors Journal compilation © 2009 Blackwell Munksgaard.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1111/j.1600-0854.2008.00873.x
dc.sourceScopus
dc.subject3D reconstruction
dc.subjectElectron microscopy
dc.subjectER exit site
dc.subjectGolgi
dc.subjectTrypanosoma brucei
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1111/j.1600-0854.2008.00873.x
dc.description.sourcetitleTraffic
dc.description.volume10
dc.description.issue3
dc.description.page300-306
dc.description.codenTRAFF
dc.identifier.isiut000263138900005
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