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Title: Transgenic Expression of Walleye Dermal Sarcoma Virus rv-cyclin (orfA) in Zebrafish does not Result in Tissue Proliferation
Authors: Paul, T.A.
Rovnak, J.
Quackenbush, S.L.
Whitlock, K.
Zhan, H. 
Gong, Z. 
Spitsbergen, J.
Bowser, P.R.
Casey, J.W.
Keywords: Fish retrovirus
Walleye dermal sarcoma
Walleye dermal sarcoma virus
Issue Date: Apr-2011
Citation: Paul, T.A., Rovnak, J., Quackenbush, S.L., Whitlock, K., Zhan, H., Gong, Z., Spitsbergen, J., Bowser, P.R., Casey, J.W. (2011-04). Transgenic Expression of Walleye Dermal Sarcoma Virus rv-cyclin (orfA) in Zebrafish does not Result in Tissue Proliferation. Marine Biotechnology 13 (2) : 142-150. ScholarBank@NUS Repository.
Abstract: Walleye dermal sarcoma (WDS) is a benign tumor of walleye fish that develops and completely regresses seasonally. The retrovirus associated with this disease, walleye dermal sarcoma virus, encodes three accessory genes, two of which, rv-cyclin (orfA) and orfb, are thought to play a role in tumor development. In this study, we attempted to recapitulate WDS development by expressing rv-cyclin in chimeric and stable transgenic zebrafish. Six stable transgenic lines expressing rv-cyclin from the constitutive CMVtk promoter were generated. Immunohistochemistry and quantitative reverse transcriptase polymerase chain reaction demonstrate that rv-cyclin is widely expressed in different tissues in these fish. These lines were viable and histologically normal for up to 2 years. No increase in tumors or tissue proliferation was observed following N-ethyl N-nitrosourea exposure or following tail wounding and subsequent tissue regeneration compared to controls. These data indicate that rv-cyclin is not independently sufficient for tumor induction in zebrafish. © 2010 Springer Science+Business Media, LLC.
Source Title: Marine Biotechnology
ISSN: 14362228
DOI: 10.1007/s10126-010-9274-2
Appears in Collections:Staff Publications

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