Please use this identifier to cite or link to this item: https://doi.org/10.1371/journal.pgen.0010029
Title: Transcriptome analysis of zebrafish embryogenesis using microarrays
Authors: Mathavan, S.
Lee, S.G.P.
Mak, A.
Miller, L.D.
Murthy, K.R.K.
Govindarajan, K.R.
Tong, Y. 
Wu, Y.L. 
Lam, S.H. 
Yang, H.
Ruan, Y.
Korzh, V.
Gong, Z. 
Liu, E.T.
Lufkin, T.
Issue Date: 2005
Citation: Mathavan, S., Lee, S.G.P., Mak, A., Miller, L.D., Murthy, K.R.K., Govindarajan, K.R., Tong, Y., Wu, Y.L., Lam, S.H., Yang, H., Ruan, Y., Korzh, V., Gong, Z., Liu, E.T., Lufkin, T. (2005). Transcriptome analysis of zebrafish embryogenesis using microarrays. PLoS Genetics 1 (2) : 0260-0276. ScholarBank@NUS Repository. https://doi.org/10.1371/journal.pgen.0010029
Abstract: Zebrafish (Danio rerio) is a well-recognized model for the study of vertebrate developmental genetics, yet at the same time little is known about the transcriptional events that underlie zebrafish embryogenesis. Here we have employed microarray analysis to study the temporal activity of developmentally regulated genes during zebrafish embryogenesis. Transcriptome analysis at 12 different embryonic time points covering five different developmental stages (maternal, blastula, gastrula, segmentation, and pharyngula) revealed a highly dynamic transcriptional profile. Hierarchical clustering, stage-specific clustering, and algorithms to detect onset and peak of gene expression revealed clearly demarcated transcript clusters with maximum gene activity at distinct developmental stages as well as coregulated expression of gene groups involved in dedicated functions such as organogenesis. Our study also revealed a previously unidentified cohort of genes that are transcribed prior to the mid-blastula transition, a time point earlier than when the zygotic genome was traditionally thought to become active. Here we provide, for the first time to our knowledge, a comprehensive list of developmentally regulated zebrafish genes and their expression profiles during embryogenesis, including novel information on the temporal expression of several thousand previously uncharacterized genes. The expression data generated from this study are accessible to all interested scientists from our institute resource database (http://giscompute. gis.a-star.edu.sg/∼govind/zebrafish/data_download.html). © 2005 Mathavan et al.
Source Title: PLoS Genetics
URI: http://scholarbank.nus.edu.sg/handle/10635/102061
ISSN: 15537390
DOI: 10.1371/journal.pgen.0010029
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