Please use this identifier to cite or link to this item: https://doi.org/10.1038/nature04184
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dc.titleThe zebrafish dorsal axis is apparent at the four-cell stage
dc.contributor.authorGore, A.V.
dc.contributor.authorMaegawa, S.
dc.contributor.authorCheong, A.
dc.contributor.authorGilligan, P.C.
dc.contributor.authorWeinberg, E.S.
dc.contributor.authorSampath, K.
dc.date.accessioned2014-10-27T08:43:45Z
dc.date.available2014-10-27T08:43:45Z
dc.date.issued2005-12-15
dc.identifier.citationGore, A.V., Maegawa, S., Cheong, A., Gilligan, P.C., Weinberg, E.S., Sampath, K. (2005-12-15). The zebrafish dorsal axis is apparent at the four-cell stage. Nature 438 (7070) : 1030-1035. ScholarBank@NUS Repository. https://doi.org/10.1038/nature04184
dc.identifier.issn00280836
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/102028
dc.description.abstractA central question in the development of multicellular organisms pertains to the timing and mechanisms of specification of the embryonic axes. In many organisms, specification of the dorsoventral axis requires signalling by proteins of the Transforming growth factor-β and Wnt families 1-3. Here we show that maternal transcripts of the zebrafish Nodal-related morphogen, Squint (Sqt), can localize to two blastomeres at the four-cell stage and predict the dorsal axis. Removal of cells containing sqt transcripts from four-to-eight-cell embryos or injection of antisense morpholino oligonucleotides targeting sqt into oocytes can cause a loss of dorsal structures. Localization of sqt transcripts is independent of maternal Wnt pathway function and requires a highly conserved sequence in the 3′ untranslated region. Thus, the dorsoventral axis is apparent by early cleavage stages and may require the maternally encoded morphogen Sqt and its associated factors. Because the 3′ untranslated region of the human nodal gene can also localize exogenous sequences to dorsal cells, this mechanism may be evolutionarily conserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1038/nature04184
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1038/nature04184
dc.description.sourcetitleNature
dc.description.volume438
dc.description.issue7070
dc.description.page1030-1035
dc.description.codenNATUA
dc.identifier.isiut000233934600070
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