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Title: The role of N and C termini in the antifreeze activity of winter flounder (Pleuronectes americanus) antifreeze proteins
Authors: Low, W.-K.
Lin, Q.
Hew, C.L. 
Issue Date: 21-Mar-2003
Citation: Low, W.-K., Lin, Q., Hew, C.L. (2003-03-21). The role of N and C termini in the antifreeze activity of winter flounder (Pleuronectes americanus) antifreeze proteins. Journal of Biological Chemistry 278 (12) : 10334-10343. ScholarBank@NUS Repository.
Abstract: Antifreeze proteins (AFPs) are found in many marine fish and have been classified into five biochemical classes: AFP types I-IV and the antifreeze glycoproteins. Type I AFPs are α-helical, partially amphipathic, Alarich polypeptides. The winter flounder (Pleuronectes americanus) produces two type I AFP subclasses, the liver-type AFPs (wflAFPs) and the skin-type AFPs (wfsAFPs), that are encoded by distinct gene families with different tissue-specific expression. wfsAFPs and wflAFPs share a high level of identity even though the wfsAFPs have approximately half the activity of the wflAFPs. Synthetic polypeptides based on two representative wflAFPs and wfsAFPs were generated to examine the role of the termini in antifreeze activity. Through systematic exchange of N and C termini between wflAFP-6 and wfsAFP-2, the termini were determined to be the major causative agents for the variation in activity levels between the two AFPs. Furthermore, the termini of wflAFP-6 possessed greater helix-stabilizing ability compared with their wfsAFP-2 counterparts. The observed 50% difference in activity between wflAF-6 and wfsAFP.2 can be divided into ∼20% for differences at each termini and ∼10% for differences in the core. Furthermore, the N terminus was determined to be the most critical component for antifreeze activity.
Source Title: Journal of Biological Chemistry
ISSN: 00219258
DOI: 10.1074/jbc.M300081200
Appears in Collections:Staff Publications

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