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|Title:||Temperature and Mg2+ sensing by a novel PhoP-PhoQ two-component system for regulation of virulence in Edwardsiella tarda||Authors:||Chakraborty, S.
|Issue Date:||10-Dec-2010||Citation:||Chakraborty, S., Li, M., Chatterjee, C., Sivaraman, J., Leung, K.Y., Mok, Y.-K. (2010-12-10). Temperature and Mg2+ sensing by a novel PhoP-PhoQ two-component system for regulation of virulence in Edwardsiella tarda. Journal of Biological Chemistry 285 (50) : 38876-38888. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M110.179150||Abstract:||The PhoP-PhoQ two-component system is commonly used by bacteria to sense environmental factors. Here we show that the PhoP-PhoQ system of Edwardsiella tarda detects changes in environmental temperature and Mg2+ concentration as well as regulates the type III and VI secretion systems through direct activation of esrB. Protein secretion is activated from 23 to 35°C or at low Mg2+ concentrations, but it is suppressed at or below 20°C, at or above 37°C, or at high Mg2+ concentrations. The effects of temperature and Mg2+ concentration are additive. The PhoQ sensor domain has a low Tm of 37.9°C, and it detects temperatures through a conformational change of its secondary structure. Mutation of specific Pro or Thr residues increased the stability of the PhoQ sensor drastically, altering its temperature-sensing ability. The PhoQ sensor detects Mg2+ concentration through the direct binding of Mg2+ to a cluster of acidic residues (DDDSAD) and through changes that likely affect its tertiary structure. Here, we describe for the first time the use of PhoP-PhoQ as a temperature sensor for bacterial virulence control. © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.||Source Title:||Journal of Biological Chemistry||URI:||http://scholarbank.nus.edu.sg/handle/10635/101835||ISSN:||00219258||DOI:||10.1074/jbc.M110.179150|
|Appears in Collections:||Staff Publications|
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