Please use this identifier to cite or link to this item: https://doi.org/10.1095/biolreprod.105.049403
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dc.titleTandem-repeated zebrafish zp3 genes possess oocyte-specific promoters and are insensitive to estrogen induction
dc.contributor.authorLiu, X.
dc.contributor.authorWang, H.
dc.contributor.authorGong, Z.
dc.date.accessioned2014-10-27T08:41:19Z
dc.date.available2014-10-27T08:41:19Z
dc.date.issued2006
dc.identifier.citationLiu, X., Wang, H., Gong, Z. (2006). Tandem-repeated zebrafish zp3 genes possess oocyte-specific promoters and are insensitive to estrogen induction. Biology of Reproduction 74 (6) : 1016-1025. ScholarBank@NUS Repository. https://doi.org/10.1095/biolreprod.105.049403
dc.identifier.issn00063363
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101818
dc.description.abstractZona pellucida (Zp) proteins are glycoproteins in fish chorion and are encoded by multiple gene families, including zp1, zp2, zp3, and potentially other zp genes. Expression of zp genes in teleosts is either in the liver under the induction of estrogen or in the ovaries. In the present study, we isolated and characterized a zebrafish zp3 genomic clone and found three tandem-repeated zp3 genes with high sequence identities. We estimated that there were 10-15 zp2 and 17-21 zp3 genes in a haploid genome. We also found some variant zp2 and zp3 subfamilies, and each subfamily may have multiple members. These zp2 and zp3 genes are distributed in several different chromosomes. Ontogenetic expression of zp2 and zp3 mRNAs was first detected at 3 wk postfertilization, which was about 5 wk earlier than initial vtg1 expression, indicating that ovary development was earlier than vitellogenesis. Both zp2 and zp3 mRNAs were expressed specifically in early-growing oocytes and are insensitive to estrogen induction. Because zp3 genes are organized in tandem repeats, to investigate whether an individual zp3 promoter is capable of driving oocyte-specific expression, green fluorescence protein (gfp)-transgenic zebrafish were developed by using a 3.8-kb zp3 5′ upstream region, and we found that the gfp reporter gene was faithfully expressed in developing oocytes in zp3:gfp transgenic females. Thus, the new transgenic line not only provided a convenient living marker for monitoring female gonad development, but also demonstrated that a single zp3 gene promoter is sufficient for oocyte-specific transcription. © 2006 by the Society for the Study of Reproduction, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1095/biolreprod.105.049403
dc.sourceScopus
dc.subjectDevelopmental biology
dc.subjectEstradiol
dc.subjectGene regulation
dc.subjectOocyte development
dc.subjectOvary
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1095/biolreprod.105.049403
dc.description.sourcetitleBiology of Reproduction
dc.description.volume74
dc.description.issue6
dc.description.page1016-1025
dc.description.codenBIREB
dc.identifier.isiut000237767900004
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