Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/101817
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dc.titleTandem repeats of Sushi3 peptide with enhanced LPS-binding and -neutralizing activities
dc.contributor.authorLi, C.
dc.contributor.authorNg, M.L.P.
dc.contributor.authorZhu, Y.
dc.contributor.authorHo, B.
dc.contributor.authorDing, J.L.
dc.date.accessioned2014-10-27T08:41:19Z
dc.date.available2014-10-27T08:41:19Z
dc.date.issued2003-08-01
dc.identifier.citationLi, C.,Ng, M.L.P.,Zhu, Y.,Ho, B.,Ding, J.L. (2003-08-01). Tandem repeats of Sushi3 peptide with enhanced LPS-binding and -neutralizing activities. Protein Engineering 16 (8) : 629-635. ScholarBank@NUS Repository.
dc.identifier.issn02692139
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101817
dc.description.abstractEndotoxin, also known as lipopolysaccharide (LPS), is the major mediator of septic shock due to Gram-negative bacterial infection. Chemically synthesized S3 peptide, derived from Sushi3 domain of Factor C, which is the endotoxin-sensitive serine protease of the limulus coagulation cascade, was previously shown to bind and neutralize LPS activity. For large-scale production of this peptide and to mimick other pathogen-recognizing molecules, tandem multimers of the S3 gene were constructed and expressed in Escherichia coli. The recombinant tetramer of S3 (rS3-4mer) was purified by anion exchange and digested into monomers (rS3-1mer). Both the rS3-4mer and rS3-1mer were functionally analyzed for their ability to bind LPS by an ELISA-based method and surface plasmon resonance. The LAL inhibition and TNFα-release test showed that rS3-1 mer can neutralize the LPS activity as effectively as the synthetic S3 peptide, while rS3-4mer displays an enhanced inhibitory effect on LPS-induced activities. Both recombinant peptides exhibited low cytotoxicity and no haemolytic activity on human cells. This evidence suggests that the recombinant sushi peptides have potential use for the detection, removal of endotoxin and/or anti-endotoxin strategies.
dc.sourceScopus
dc.subjectEndotoxin binding and neutralization
dc.subjectFactor C
dc.subjectLimulus
dc.subjectS3 tandem repeats
dc.subjectSushi3 peptide
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.sourcetitleProtein Engineering
dc.description.volume16
dc.description.issue8
dc.description.page629-635
dc.description.codenPRENE
dc.identifier.isiutNOT_IN_WOS
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