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Title: Succinic semialdehyde couples stress response to quorum-sensing signal decay in Agrobacterium tumefaciens
Authors: Wang, C.
Zhang, H.-B.
Wang, L.-H.
Zhang, L.-H. 
Issue Date: Oct-2006
Citation: Wang, C., Zhang, H.-B., Wang, L.-H., Zhang, L.-H. (2006-10). Succinic semialdehyde couples stress response to quorum-sensing signal decay in Agrobacterium tumefaciens. Molecular Microbiology 62 (1) : 45-56. ScholarBank@NUS Repository.
Abstract: Quorum sensing (QS) signal decay in Agrobacterium tumefaciens occurs in response to starvation or host signals. We have demonstrated that the γ-aminobutyric acid (GABA) shunt metabolite links stress response to QS signal decay. Mutation of the aldH gene encoding a succinic semialdehyde dehydrogenase (SSADH) that converts succinic semialdehyde (SSA) to succinic acid results in early expression of the signal degrading enzyme, AttM. Exogenous addition of SSA or its precursor GABA induces AttM expression and abolishes Ti plasmid conjugative transfer. SSA acts by binding to the repressor AttJ that regulates the attKLM operon. attK encodes another SSADH. The stress alarmone ppGpp and SSA modulates separately the expression of the two SSADH enzymes, which might control the intracellular SSA level and hence to switch on/off the QS signal decay system in response to environmental changes. These findings document for the first time a sophisticated signalling mechanism of the widely conserved GABA degradation pathway in prokaryotes. © 2006 The Authors.
Source Title: Molecular Microbiology
ISSN: 0950382X
DOI: 10.1111/j.1365-2958.2006.05351.x
Appears in Collections:Staff Publications

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