Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/101785
Title: Sub-population analysis based on temporal features of high content images
Authors: Veronika, M.
Evans, J.
Matsudaira, P. 
Welsch, R.
Rajapakse, J.
Issue Date: 3-Dec-2009
Citation: Veronika, M.,Evans, J.,Matsudaira, P.,Welsch, R.,Rajapakse, J. (2009-12-03). Sub-population analysis based on temporal features of high content images. BMC Bioinformatics 10 (SUPPL. 15) : -. ScholarBank@NUS Repository.
Abstract: Background: High content screening techniques are increasingly used to understand the regulation and progression of cell motility. The demand of new platforms, coupled with availability of terabytes of data has challenged the traditional technique of identifying cell populations by manual methods and resulted in development of high-dimensional analytical methods. Results: In this paper, we present sub-populations analysis of cells at the tissue level by using dynamic features of the cells. We used active contour without edges for segmentation of cells, which preserves the cell morphology, and autoregressive modeling to model cell trajectories. The sub-populations were obtained by clustering static, dynamic and a combination of both features. We were able to identify three unique sub-populations in combined clustering. Conclusion: We report a novel method to identify sub-populations using kinetic features and demonstrate that these features improve sub-population analysis at the tissue level. These advances will facilitate the application of high content screening data analysis to new and complex biological problems. © 2009 Veronika et al; licensee BioMed Central Ltd.
Source Title: BMC Bioinformatics
URI: http://scholarbank.nus.edu.sg/handle/10635/101785
ISSN: 14712105
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

79
checked on Aug 1, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.