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https://doi.org/10.1016/j.jsb.2011.08.007
Title: | Structural characterization of BVU_3255, a methyltransferase from human intestine antibiotic resistant pathogen Bacteroides vulgatus | Authors: | Kumar, V. Sivaraman, J. |
Keywords: | Antibiotic resistance Bacteroides vulgatus BVU_3255 Crystal structure SAH SAM Small molecule methyltransferase |
Issue Date: | Dec-2011 | Citation: | Kumar, V., Sivaraman, J. (2011-12). Structural characterization of BVU_3255, a methyltransferase from human intestine antibiotic resistant pathogen Bacteroides vulgatus. Journal of Structural Biology 176 (3) : 409-413. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jsb.2011.08.007 | Abstract: | Methylation is important for various cellular activities. To date, there is no report of any methyltransferase structure from the human intestine antibiotic resistant pathogen Bacteroides vulgatus. The protein BVU_3255 from B. vulgatus ATCC 8482 belongs to a SAM-dependent methyltransferase. Here, we report the crystal structure of apo BVU_3255, and its complexes with SAM and SAH, which revealed a typical class I Rossmann Fold Methyltransferase. Isothermal titration calorimetric studies showed that both SAM and SAH bind with equal affinity. The structural and sequence analysis suggested that BVU_3255 is a small molecule methyltransferase and involved in methylating the intermediates in ubiquinone biosynthesis pathway. © 2011 Elsevier Inc. | Source Title: | Journal of Structural Biology | URI: | http://scholarbank.nus.edu.sg/handle/10635/101757 | ISSN: | 10478477 | DOI: | 10.1016/j.jsb.2011.08.007 |
Appears in Collections: | Staff Publications |
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