Please use this identifier to cite or link to this item: https://doi.org/10.1038/emboj.2008.278
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dc.titleStructural basis for translational inhibition by the tumour suppressor Pdcd4
dc.contributor.authorLoh, P.G.
dc.contributor.authorYang, H.-S.
dc.contributor.authorWalsh, M.A.
dc.contributor.authorWang, Q.
dc.contributor.authorWang, X.
dc.contributor.authorCheng, Z.
dc.contributor.authorLiu, D.
dc.contributor.authorSong, H.
dc.date.accessioned2014-10-27T08:40:37Z
dc.date.available2014-10-27T08:40:37Z
dc.date.issued2009-02-04
dc.identifier.citationLoh, P.G., Yang, H.-S., Walsh, M.A., Wang, Q., Wang, X., Cheng, Z., Liu, D., Song, H. (2009-02-04). Structural basis for translational inhibition by the tumour suppressor Pdcd4. EMBO Journal 28 (3) : 274-285. ScholarBank@NUS Repository. https://doi.org/10.1038/emboj.2008.278
dc.identifier.issn02614189
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101752
dc.description.abstractPdcd4 is a tumour suppressor protein. It inhibits translation through interaction with translation initiator eIF4A, resulting in the suppression of neoplastic transformation and tumour invasion. Here, we present the crystal structures of an N-terminal-truncated Pdcd4 in free form and in complex with eIF4A. Upon binding to eIF4A, Pdcd4 undergoes a marked conformational change to form a heterotrimeric complex with eIF4A, with one Pdcd4 binding to two eIF4A molecules in two different modes. The binding of Pdcd4 to eIF4A is required to inhibit the enzymatic activity of eIF4A, translation initiation, and AP-1-dependent transcription. Both MA3 domains are required to efficiently compete with the C-terminal domain of eIF4G (eIF4Gc) for binding to eIF4A whereas a single MA3 is sufficient to inhibit translation. Our structural and mutational analyses reveal that Pdcd4 inhibits translation initiation by trapping eIF4A in an inactive conformation, and blocking its incorporation into the eIF4F complex. © 2009 European Molecular Biology Organization | All Rights Reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1038/emboj.2008.278
dc.sourceScopus
dc.subjectPdcd4
dc.subjectRNA helicase
dc.subjectTranslation initiation
dc.subjectTranslational control
dc.subjectTumour suppressor
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1038/emboj.2008.278
dc.description.sourcetitleEMBO Journal
dc.description.volume28
dc.description.issue3
dc.description.page274-285
dc.description.codenEMJOD
dc.identifier.isiut000263075900014
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