Resolution-enhanced 4D 15N/13C NOESY protein NMR spectroscopy by application of the covariance transform

Abstract

The combination of shared-evolution 4D 15N/13C-edited NOESY spectroscopy with covariance NMR is introduced, which yields, as a sub-spectrum, an asymmetric 4D 15N/13C-edited NOESY spectrum at a resolution enhanced 5-fold over the one of a non-shared 4D 15N/13C-edited NOESY measured with the same sweep widths and number of increments. The achieved resolution enhancement allows for a substantial increase in the number of assigned NOEs over that of the 4D Fourier transform spectrum and should prove useful for efficient, high-resolution NMR-based studies of protein structure. Copyright © 2007 American Chemical Society.