Please use this identifier to cite or link to this item: https://doi.org/10.1023/A:1018307118879
Title: Recombinant COS-1 cells express Carcinoscorpius rotundicauda Factor C
Authors: Dwarakanath, R.S.
Ho, B.
Ding, J.L. 
Issue Date: 1997
Citation: Dwarakanath, R.S., Ho, B., Ding, J.L. (1997). Recombinant COS-1 cells express Carcinoscorpius rotundicauda Factor C. Biotechnology Letters 19 (4) : 357-361. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1018307118879
Abstract: The cDNA encoding Carcinoscorpius rotundicauda Factor C, CrFC, when expressed in mammalian cells, produced full length and truncated variants of rFC. In an in vitro coupled transcription-translation system, aberrant products were generated from pCDNAI/CrFC21, as opposed to pCIneo/CrFC21 which gave rise to the expected polypeptide. COS-1 cells transfected with pCIneo/CrFC21 expressed Factor C-related proteins which differ from those produced in the yeast, Pichia pastoris. The expression of a complex, mosaic protein such as the rFC in mammalian cells also resembled other mammalian blood factors, indicating that such cells could be used for production of a second generation 'limulus lysate'.
Source Title: Biotechnology Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/101537
ISSN: 01415492
DOI: 10.1023/A:1018307118879
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